探讨前颌骨矫正对上颌骨生长发育的影响；检测上颌骨周围 骨缝成骨的活跃程度。方法：以40 d Wistar大鼠建立人 工双侧腭裂模型，Latham矫正器前颌骨矫 正；应用免疫组化和原位杂交技术检测上颌骨周围骨缝TGF-β的表达。结果： 免疫组化检 测，矫正第7，14 天后实验组大鼠TGF-β的阳性表达与两对照组之间无显著性差异；原位 杂交检测，从第7 天开始实验组TGF-β mRNA阳性表达明显高于对照组，两对照组之间差异无显著 性。结论：前颌骨矫正后，大鼠上颌骨周围骨缝区有成骨活跃。
中图分类号：R782.2+2 文献标识码：A
文章编号：1001-3733(2000)03-0188-04
The effects of premaxillary orthopedics on the expression
of transforming growth factor in artificial bilateral cleft palate of Wistar rats
Fang Bing,Qiu Weiliu,Yuan Wenhua,et al.
(Department of Oral and Maxillofacial Surg ery,Shanghai Second
Medical University Affiliate 9thPeople's Hospital, Sha nghai 200011)
〔Abstract〕Objective:To determine the expression of transforming (TG F-β) in t he jugomaxillary sutures,temporomalar sutures and sphenopalatine sutures after p r emaxillary orthopedics.Methods:Immunohistochemistry techni que and situ hybridiza tion technique were used to detect the expression of TGF-β among the subject g roup and tow control groups.Results: TGF-β positive reacti on was not different among the three gropes after orthopedics detected by immunohistochemistry (P ＞0.0 5) in 14 days.The level of TGF-β mRNA in the subject groupe was higher than th at in th e control after 7～14 days treatment.Conclusion: Orthopedi
